ELISA Microplates allow a standard laboratory test to be performed on numerous samples at the same time.
8-well strips and 96-well plates are popular formats. In terms of identifying protein species, ELISA tests are the gold standard. They are useful for analyzing protein mixtures or confirming the presence of a specific protein.
Because of its sensitivity to low abundance proteins, ELISA is increasingly being employed in laboratory examination of blood samples. Plates are often prepared with chemicals to ensure optimum protein binding. The needs of the plate reader and the detection technique should be considered while selecting ELISA microplates. Although most microplates now meet global standards, it is a good idea to double-check the specs to ensure compatibility with your reader and chemicals.
The Elisa plate is mainly divided into detachable and non-detachable. Among them, the transparent plate is suitable for quantitative and qualitative solid-phase immunodetection and binding detection; the black plate is suitable for fluorescence immunoassay and binding detection; the white plate is suitable for self-luminescence and chemiluminescence, and is suitable for most standard equipment.
The protein adsorption capacity of the ELISA plate is divided into three levels:
Low binding capacity:
preferentially attach to molecules with large hydrophobic regions. (lipids, lipoproteins, large proteins)
Medium binding capacity:
binding to biomolecules with hydrophilic/hydrophobic properties (medium and large protein, immunoglobulin, albumin)
High binding capacity:
preferentially bind biomolecules with hydrophilic/ hydrophobic properties (small to large proteins, immunoglobulins, albumin, LPS, proteins)