CTnI / CK-MB /Myo POC Test Kit TRFIA Immunoassay High Sensitivity

CTnI/CK-MB/Myo POC Test Kit TRFIA Immunoassay Cardiac Troponin I / Creatine Kinase Isoenzyme / Myoglobin Myo Test Kit

High sensitivity cFDA approved cTnI/CK-MB/MYO Quantitative Detection Kit (TRFIA)

This cTnI/CK-MB/MYO Test Kit (Time-Resolved Fluorescence Immunochromatographic Assay) is suitable for quantitative detection of cardiac troponin I, creatine kinase isoenzyme, and myoglobin in human serum/plasma /whole blood in vitro.
The measurement of cardiac troponin I (cTnI) may help in the diagnosis of an acute myocardial infarction (AMI). Clinical investigations have shown that cardiac troponin I may be identified in the blood 4-6 hours after an AMI and can stay high for many days. Because of its myocardial specificity and sensitivity, cTnI may be utilized as a gold standard for diagnosing AMI.
Creatine kinase isoenzyme (CK-MB) is widely used as a diagnostic marker of myocardial injury in the detection of acute myocardial infarction (AMI). Clinical studies have shown that CK-MB is released into blood circulation during myocardial injury and peaks within 12~24 hours and returns to normal level within 48~72 hours. Therefore, the detection of CK-MB has great clinical significance for the diagnosis of AMI.
Myoglobin (abbreviated MYO) is an early indicator of myocardial necrosis. Myoglobin may be found in clinical tests within 1 hour after start and peaks in 45 hours. However, myoglobin levels increase in several situations, including skeletal muscle damage, skeletal muscle or neuromuscular dysfunction, cardiac shunt surgery, renal failure, and high-intensity physical activity. As a result, myoglobin elevation must be utilized as an auxiliary diagnosis of acute myocardial infarction (AMI) with other cardiac markers, electrocardiogram (ECG), clinical symptoms and signs, and so on. Chemiluminescence, enzyme immunoassay, fluorescence immunoassay, and colloidal gold assay are now used to detect the levels of cTnI, CK-MB, and MYO in blood samples.
 

The cTnI/CK-MB/MYO Test Kit (Time-Resolved Fluorescence Immunochromatographic Assay) is based on time-resolved fluorescence immunochromatography. The concentration of cTnI, CK-MB, and MYO in blood samples was quantitatively determined. The samples were added to the reagent card, and the cTnI/CK-MB/MYO of the sample was combined with antibodies (mouse anti-human cTnI monoclonal antibody, mouse anti-human CK-MB monoclonal antibody, and mouse anti-human MYO monoclonal antibody) labeled nanomicrosphere fluorescent probe to form a complex (cTnI/CK-MB/MYO-monoclonal antibody labeled nanomicrosphere fluorescent probe). Through capillarity, the complex was captured by antibodies (mouse anti-human cTnI monoclonal antibody, mouse anti-human CK-MB monoclonal antibody, and mouse anti-human MYO monoclonal antibody ) on the nitrocellulose membrane, and form double antibody sandwich complex(monoclonal antibody--cTnI/CK-MB/MYO--monoclonal antibody labeled nanomicrosphere fluorescent probe).As a result, the more cTnI/CK-MB/MYO in the sample, the more the double antibody sandwich complex accumulates on the detection line. The excess unreacted antibodies labeled nanomicrosphere fluorescent probe continued to chromatography to the control line. The fluorescence intensity on the detection line is positively correlated with the concentration of the object (cTnI/CK-MB/MYO) in the sample, and the regression equation is calculated and written into the IC card.
The time-resolved fluorescence immunoanalyzer reads the regression equation data from the IC card, tests the fluorescence intensity on the detection line, and then automatically calculates the concentration of the object (cTnI/CK-MB/MYO) in the output sample using the fluorescence intensity as a substitute.
 
 

High sensitivity cFDA approved cTnI/CK-MB/MYO Quantitative Detection Kit (TRFIA) Main component

• cTnI/CK-MB/MYO Test Kit (Time Resolved Fluorescence Immunochromatographic Assay) :Contains 20/50 reagent cards ,1 IC card ,1 instruction for use ,20 /50 sample buffers.
• The reagent card consists of plastic casings and test strips. Test strips are made of sample pad, marker pad(sprayed by mouse anti-human cTnI monoclonal antibody nano-microspheres fluorescent probe[0.2,0.3] mg/mL, mouse anti-human CK-MB monoclonal antibody nano-microspheres fluorescent probe[0.15,0.25] mg/mL,mouse anti-human MYO monoclonal antibody nano-microspheres fluorescent probe[0.2,0.3] mg/mL), Detection of membranes(sprayed by mouse anti-human cTnI monoclonal antibody [1.5,2.0] mg/mL, mouse anti-human CK-MB monoclonal antibody [1.0,1.5] mg/mL, mouse anti-human MYO monoclonal antibody [1.0,1.5] mg/mL, Polyclonal antibody against mouse immunoglobulin G (Ig G) in sheep[0.3,0.7] mg/mL), absorbent paper and polystyrene (PVC) plywood. Sample Buffer,the main component is saline(0.9% NaCl).
• ※Do not exchange different batch numbers

Storage conditions and expiry date?
The unopened kit was kept at 2°C8°C for 18 months. If the expiration date written on the packaging is not surpassed, the product may be kept at room temperature (20°C-30°C) for 30 days. Temperature fluctuations during transit (37°C) have no influence on product quality. To detect a blood sample, the reagent strip and sample buffer are used in one-to-one mode, which means that a reagent strip is matched with a sample buffer. It may be stabilized at room temperature for 1 hour after unsealing. Store and use in accordance with the aforementioned specifications under normal humidity and light conditions.