|High Sensitivity CTnI/CK-MB/MYO Quantitative Detection Kit （TRFIA）
|Whole Blood, Plasma, Serum
|Place Of Origin:
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High sensitivity cFDA approved cTnI/CK-MB/MYO Quantitative Detection Kit (TRFIA)
1. Product Description
The cTnI/CK-MB/MYO Test Kit (Time Resolved Fluorescence Immunochromatographic Assay) is based on time-resolved fluorescence immunochromatography. The concentration of cTnI, CK-MB and MYO in blood samples was quantitatively determined. The samples were added to the reagent card, and the cTnI/CK-MB/MYO of the sample was combined with antibodies (mouse anti-human cTnI monoclonal antibody, mouse anti-human CK-MB monoclonal antibody and mouse anti-human MYO monoclonal antibody) labeled nanomicrosphere fluorescent probe to form complex (cTnI/CK-MB/MYO-monoclonal antibody labeled nanomicrosphere fluorescent probe). Through capillarity, the complex was captured by antibodies (mouse anti-human cTnI monoclonal antibody, mouse anti-human CK-MB monoclonal antibody and mouse anti-human MYO monoclonal antibody ) on nitrocellulose membrane, and form double antibody sandwich complex(monoclonal antibody--cTnI/CK-MB/MYO--monoclonal antibody labeled nanomicrosphere fluorescent probe).As a result, the more cTnI/CK-MB/MYO in the sample, the more the double antibody sandwich complex accumulates on the detection line. The excess unreacted antibodies labeled nanomicrosphere fluorescent probe continued to chromatography to the control line.The fluorescence intensity on the detection line is positively correlated with the concentration of the object (cTnI/CK-MB/MYO) in the sample, and the regression equation is calculated and written into the IC card.
The time-resolved fluorescence immunoanalyzer reads the regression equation data in the IC card, tests the fluorescence intensity on the detection line, and then replaces the fluorescence intensity with the regression equation for automatic calculation, that is, the concentration of the object (cTnI/CK-MB/MYO) in the output sample.
This cTnI/CK-MB/MYO Test Kit (Time Resolved Fluorescence Immunochromatographic Assay) is suitable for quantitative detection of cardiac troponin I, creatine kinase isoenzyme and myoglobin in human serum/plasma /whole blood in vitro.
The determination of cardiac troponin I (hereinafter as cTnI) can be used to assist in the diagnosis of acute myocardial infarction (AMI).Clinical studies have confirmed that cardiac troponin I can be detected in the blood 4-6 hours after acute myocardial infarction (AMI) and can remain elevated for several days. cTnI can be used as a gold standard for diagnostic AMI due to its myocardial specificity and sensitivity.Creatine kinase isoenzyme (CK-MB) is widely used as a diagnostic marker of myocardial injury in the detection of acute myocardial infarction (AMI). Clinical studies have shown that CK-MB is released into blood circulation during myocardial injury and peaks within 12~24 hours and returns to normal level within 48~72 hours.Therefore, the detection of CK-MB has great clinical significance for the diagnosis of AMI. Myoglobin (abbreviated as MYO below) is an early marker of myocardial necrosis. Clinical studies have shown that myoglobin can be detected within 1 hour of onset and peaks in 4~5 hours.But in many cases myoglobin levels rise, such as skeletal muscle injury, skeletal muscle or neuromuscular dysfunction, cardiac shunt surgery, renal failure, and high-intensity physical exercise. Therefore, the elevation of myoglobin must be used in the auxiliary diagnosis of acute myocardial infarction (AMI) together with the detection of other cardiac markers, electrocardiogram (ECG), clinical symptoms and signs, etc.Currently,common methods of detecting the levels of cTnI, CK-MB and MYO in blood samples are chemiluminescence, enzyme immunoassay, fluorescence immunoassay and colloidal gold assay, etc.
|High sensitivity cFDA approved cTnI/CK-MB/MYO Quantitative Detection Kit (TRFIA)
|Whole blood, Plasma, Serum
|Place of Origin
|Suzhou, Jiangsu, China
|Negotiable, Accept small quantity
|Depends on the order, 1~15 working days
|Best to inquire for shipping details. We will contact you within 24 hours. Door to door service can be provided in some areas.
3. High sensitivity cFDA approved cTnI/CK-MB/MYO Quantitative Detection Kit (TRFIA) Main component
4. Storage conditions and expiry date?
The unopened kit was stored in 2℃~8℃ for 18 months. The product can be stored at temperature (20℃~30℃) for 30 days if expiry printed on the package is not exceeded. The temperature change during transportation (<37℃) has no effect on product quality. The reagent strip and the sample buffer are used in one-to-one mode, that is, a reagent strip is matched with a sample buffer to detect a blood sample.After unsealing, it can be stabilized for 1 hour at room temperature. Under normal humidity and light conditions, store and use according to the above requirements.
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