Heart Type Fatty Acid Binding Protein H-FABP Test Kit (TRFIA)

The H-FABP Test Kit is suitable for quantitative determination of H-FABP in human whole blood/serum/plasma in vitro.

Cardiac fatty acid binding protein can not only be used as an early marker of AMI, but also an ideal diagnostic marker for recurrence of myocardial infarction. At present, the application of cardiac fatty acid binding protein mainly includes the following aspects: early diagnosis AMI evaluation of myocardial infarction area, evaluation of early myocardial microinjury, evaluation of myocardial ischemia-reperfusion, evaluation of prognosis of heart failure, etc. Based on time-resolved fluorescence technique, the H-FABP content in human whole blood/serum/plasma was determined by double antibody sandwich immunochromatography. The detection platform of this project mainly includes chemiluminescence technology, immunocompetency technology, colloidal gold technology, immunofluorescence technology, time-resolved fluorescence technology and so on.

Principle

Cardiac fatty acid binding protein detection kit is a time-resolved fluorescence immunochromatographic technique for quantitative determination of central fatty acid binding protein (H-FABP) in blood samples. The sample was added to the reagent card. The sample H-FABP coupled with the fluorescent microspheres on the marker pad to form a complex against human H-FABP monoclonal antibody. The detection band containing another specific antibody H-FABP nitric acid fiber membrane was detected by capillary action chromatography and captured to form a double antibody sandwich complex. As a result, the more H-FABP in the sample, the more complex accumulation on the detection band. The excess unreacted fluorescent microspheres continued to be chromatographic to the control line. After the reagent strip is excited, the fluorescence intensity on the detection line reflects the content of the captured H-FABP in the sample. The concentration of H-FABP in the sample was quantitatively reflected by the LTRIC-300/ LTRIC-600/LTRIC-1000 of time-resolved fluorescence immunoassay.